Food allergy and anaphylaxis – 2043. Poor dilutional linearity of food allergen specific IgE measurement by immunocap in samples with high total IgE
نویسندگان
چکیده
Methods 31 deidentified subjects with tIgE 1000-5000 kU/L (Group A) and 10 subjects with tIgE >5000 kU/L (Group B) had sIgE measurement for egg, milk, wheat, peanut, and soybean by ImmunoCap from undiluted and 4 serial two-fold dilutions using manufacturer supplied diluent. Myeloma IgE was prepared at an initial dilution of 1:28 and then 7 serial two-fold dilutions were performed. tIgE percentage recovery was calculated by multiplying measured tIgE by the dilution factor for that sample and dividing this result by the measured tIgE in neat serum. The same calculation was applied to sIgE.
منابع مشابه
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عنوان ژورنال:
دوره 6 شماره
صفحات -
تاریخ انتشار 2013